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KMID : 0545119950050050302
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 5 p.302 ~ p.304
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A New HPLC Condition for the Analysis of Aclainomycins A and Y in the Mixtures of Aclacinomycins
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Kim, Wan Seop
Cho, Won Tae/Kim, Myung Kook/Rhee, Sang Ki/Kim, Hak Ryul/Choi, Eui Sung
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Abstract
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As the previous HPLC method (Ogasawara et al., 1983) was described for the analysis of aclacinomycin A and some of its analogue compounds but not for aclacinomycin Y, we developed novel HPLC condition by optimizing solvent system. The newly developed solvent system allowed a complete separation of aclacinomycins Y and A, as opposed to the incomplete resolution of these two compounds in conventional method. The amounts of aclacinomycins Y and A could be accurately determined when the fermentation broth of Streptomyces lavendofoliae DKRS was analyzed by the newly developed method.
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